Glyko Enzymes

Our selection of enzymes includes highly purified exoglycosidases, endoglycosidases and glycosyltransferases. These enzymes are useful in both released glycan assays as well as sequencing studies when used in combination.

Glyko® PNGase F (native), 100 mU [GKE-5003]

Glyko® PNGase F (native), 100 mU [GKE-5003]

$ Inquire

Native PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc...

N-Glycanase® (PNGase F), 100 mU [GKE-5006A]

N-Glycanase® (PNGase F), 100 mU [GKE-5006A]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F), 200 mU [GKE-5006B]

N-Glycanase® (PNGase F), 200 mU [GKE-5006B]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F), 1 U [GKE-5006D]

N-Glycanase® (PNGase F), 1 U [GKE-5006D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase-PLUS® (PNGase F), 400 mU [GKE-5010B]

N-Glycanase-PLUS® (PNGase F), 400 mU [GKE-5010B]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase-PLUS® (PNGase F), 1 U [GKE-5010D]

N-Glycanase-PLUS® (PNGase F), 1 U [GKE-5010D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 100 mU [GKE-5016A]

N-Glycanase® (PNGase F, EDTA-Free), 100 mU [GKE-5016A]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 200 mU [GKE-5016B]

N-Glycanase® (PNGase F, EDTA-Free), 200 mU [GKE-5016B]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® (PNGase F, EDTA-Free), 1 U [GKE-5016D]

N-Glycanase® (PNGase F, EDTA-Free), 1 U [GKE-5016D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® ULTRA (PNGase F, EDTA-Free), 400 mU [GKE-5020B]

N-Glycanase® ULTRA (PNGase F, EDTA-Free), 400 mU [GKE-5020B]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

N-Glycanase® ULTRA (PNGase F, EDTA-Free), 1 U [GKE-5020D]

N-Glycanase® ULTRA (PNGase F, EDTA-Free), 1 U [GKE-5020D]

$ Inquire

PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase. Releases intact N-glycans by cleaving between the innermost GlcNAc and...

Glyko® PNGase A, 2 mU [GKE-5011A]

Glyko® PNGase A, 2 mU [GKE-5011A]

$ Inquire

Cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in...

Glyko® PNGase A, 10 mU [GKE-5011B]

Glyko® PNGase A, 10 mU [GKE-5011B]

$ Inquire

Cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in...

Glyko® Endo H [GKE-5002]

Glyko® Endo H [GKE-5002]

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Cleaves within the N,N'-diacetylchitobiose (GlcNAc-GlcNAc) core of high mannose and some hybrid type N-glycans. Endoglycosidase...

Glyko® Endo F2 [GKE-5008]

Glyko® Endo F2 [GKE-5008]

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Cleaves within the chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-glycans. Endoglycosidase...

O-Glycanase™ [GK80090]

O-Glycanase™ [GK80090]

$ Inquire

Releases unsubstituted, Ser/Thr-linked GalGalNAc from proteins; used with additional enzymes to remove more complex...

Glyko® Sialidase A™ [GK80040]

Glyko® Sialidase A™ [GK80040]

$ Inquire

Releases α(2,3)-, α(2,6)-, α(2,8)-, and α(2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic...

Glyko® Sialidase A™ 51 [GK80045]

Glyko® Sialidase A™ 51 [GK80045]

$ Inquire

Lower MW version of GK80040 sialidase A for challenging digestions. Releases α(2-3)-, α(2-6)-, α(2-8), and...

Glyko® Sialidase A™ 66 [GK80046]

Glyko® Sialidase A™ 66 [GK80046]

$ Inquire

Lower MW version of GK80040 sialidase A for challenging digestions. Releases α(2-3)-, α(2-6)-, α(2-8), and...

Glyko® Sialidase A™ Sampler Kit [GK80043]

Glyko® Sialidase A™ Sampler Kit [GK80043]

$ Inquire

Try our lower MW sialidase A isoforms for challenging sialidase digests. This Sampler Kit contains...

Glyko® Sialidase C™ [GK80030]

Glyko® Sialidase C™ [GK80030]

$ Inquire

Releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Source:Recombinant from Clostridium perfringens, expressed...

Glyko® Sialidase V™ [GKX-5020]

Glyko® Sialidase V™ [GKX-5020]

$ Inquire

Releases α(2-3,6,8)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Figure 1: Cleavage specificity...

Glyko® α(1-3,4,6)-Galactosidase [GKX-5007]

Glyko® α(1-3,4,6)-Galactosidase [GKX-5007]

$ Inquire

The enzyme releases non-reducing terminal α(1-3,4,6)-linked galactose from oligosaccharides. Source: Green coffee bean. NOTE:...

Glyko® β(1-4,6)-Galactosidase [GKX-5012]

Glyko® β(1-4,6)-Galactosidase [GKX-5012]

$ Inquire

Hydrolyses non-reducing terminal Galβ(1-6)GlcNAc and Galβ(1-4)GlcNAc. Galβ(1-3)GlcNAc is hydrolyzed very slowly. The relative rates...

Glyko® β(1-3,4)-Galactosidase [GKX-5013]

Glyko® β(1-3,4)-Galactosidase [GKX-5013]

$ Inquire

Hydrolyzes non-reducing terminal galactose β(1-3) and β(1-4) linkages. Can be used in conjunction with...

Glyko® β(1-4)-Galactosidase [GKX-5014]

Glyko® β(1-4)-Galactosidase [GKX-5014]

$ Inquire

The enzyme releases non-reducing terminal β(1-4)-linked galactose from oligosaccharides and glycoproteins [1]. This specificity...

Glyko® β N-Acetylhexosaminidase [GKX-5003]

Glyko® β N-Acetylhexosaminidase [GKX-5003]

$ Inquire

Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Source:Jack bean. Specificity:The enzyme...

Glyko® β N-Acetylhexosaminidase [GK80050]

Glyko® β N-Acetylhexosaminidase [GK80050]

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Cleaves all non-reducing terminal β-linked N-acetylglucosamine (GlcNAc). Also known as N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase. β(1-2,3,4,6)-specific. Use...

Glyko® β (1-2,3,4,6) Hexosaminidase, Sequencing-grade [GKX-5023]

Glyko® β (1-2,3,4,6) Hexosaminidase, Sequencing-grade [GKX-5023]

$ Inquire

Releases non-reducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Supplied at a higher concentration than...

Glyko® α(1-2)-Mannosidase [GKX-5009]

Glyko® α(1-2)-Mannosidase [GKX-5009]

$ Inquire

Releases α(1-2)-linked mannose residues from the non-reducing terminus of oligosaccharides. Used in exoglycosidase sequencing....

Glyko® α(1-2,3,6)-Mannosidase [GKX-5010]

Glyko® α(1-2,3,6)-Mannosidase [GKX-5010]

$ Inquire

Releases non-reducing terminal α(1-2,3,6)-linked mannose from oligosaccharides. Source: Jack bean. Specificity: The enzyme has...

Glyko® α(1-2,3,4,6) Fucosidase [GKX-5006]

Glyko® α(1-2,3,4,6) Fucosidase [GKX-5006]

$ Inquire

Broad specificity; releases non-reducing terminal α(1-2,3,4,6)-linked fucose from N- and O-glycans. Cleaves α(1-6) core fucose (linked...

β(1,4)-Galactosyltransferase [GKT-GA14]

β(1,4)-Galactosyltransferase [GKT-GA14]

$ Inquire

For in vitro galactosylation of glycoproteins such as monoclonal antibodies. Recombinant β(1,4)-Galactosyltransferase (B4GalT1, EC...

α(2,6)-Sialyltransferase [GKT-S26]

α(2,6)-Sialyltransferase [GKT-S26]

$ Inquire

For in vitro sialylation of glycoproteins such as monoclonal antibodies. Recombinant α(2,6)-Sialyltransferase (ST6Gal1, EC 2.4.99.1)...

Glyko® Sialidase S™ [GK80021]

Glyko® Sialidase S™ [GK80021]

$ Inquire

Sialidase S, releases α(2-3)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Replaces Glyko® Sialidase S™...

α(2,3)-Sialyltransferase [GKT-S23]

α(2,3)-Sialyltransferase [GKT-S23]

$ Inquire

For in vitro sialylation of glycoproteins such as monoclonal antibodies. Recombinant α(2,3)-Sialyltransferase (ST3Gal6, EC 2.4.99.4)...