The new on-matrix 2-AB Express™ labeling method developed by ProZyme eliminates the lengthy dry down step of traditional 2-AB protocols. Combined with rapid deglycosylation and cleanup, samples are ready for analysis in ~2.5 hours total time.
The Gly-X™ N-Glycan Rapid Release and Labeling with 2-AB Express™ kit utilizes a novel 5-minute in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. After a simple clean-up step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC-MS, MS/MS and other methods.