Gly-X is a next generation N-glycan sample preparation platform that provides a simplified in-solution workflow.
Rapid (5-minute) release of N-glycans with PNGase F combined with InstantDyes and an efficient vacuum plate cleanup step to remove excess label and denaturant allows labeled N-glycan samples to be ready for analysis in 1 hour or less for InstantDyes, or 2.5 hours with 2-AB Express.
Four dyes are currently available for the Gly-X platform:
InstantPC, a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.Product Guide
2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2.5 hours. Product Guide
InstantAB, ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.Product Info
APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours. Product Info
InstantQ, a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.Product Guide